PPT Slide

Expressed Sequence Tag (EST) Libraries

1) Create cDNA library

mRNA

cDNA

Package

Propagate

2) Sequence EST’s

Typical Library

• 300-500 bp per sequence

• 3’ region of transcript

• Depth of library depends on

number of sequences

• Database of sequences

3) Virtual Northerns

• Identify the presence and abundance of

sequences in multiple EST libraries

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